Module 9 2024

03/09/2024

Assay Design & Stats Analysis

Precision increased by: • Controlling or reducing factors causing variability e.g cell number, temperature. • Increasing replicates within an assay (e.g. n=2 or n=3) and number of assays which produce a reportable result • It’s not possible to control all variables – try to identify the most influential. • Also, practically there are limitations on the number of replicates and assays to be run.

Accuracy improved by: • Reducing sources of bias e.g. even seeding of cells within an assay plate.

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Measurements in Bioassays

• Without functional similarity

standard

sample

response

log dose

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