Module 9 2024

03/09/2024

Advancements in Technologies I

Ready-to-use cells vs Continuous cell culture

Ready to use: • a single uniform large bank of e.g. 200 vials prepared and frozen at single passage so as to use e.g. 1 vial per assay. • Storage space for many more vials required initially • allows confidence that assay cells will not change response at older passages. • Not all cells respond to thaw and use conditions.

Continuous cell culture: • Requires weekly resource for maintaining cells in culture. • Contamination of cultures mean downtime to get back to assay

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Advancements in Technologies II

• Reporter gene assays – continually improving to meet current market needs, specifically engineered for signal transduction of specific receptors, also for multi cell type assays e.g. T-cell activation assays. • Automation – can be labour intensive initially to understand programming of instruments, in the long term increases productivity and consistency e.g. liquid handlers FeliX, Echo.

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