Module 9 2024

03/09/2024

Other Assays & Platforms

Label-Free Biomolecular Interaction Analysis:

Biacore (Surface Plasmon Resonance - SPR)

Octet (Bio-Layer Interferometry - BLI)

Bead-based assays:

Luminex (bead-based multiplexing technology)

AlphaLisa (bead-based, no-wash solution phase assay)

Delfia (Time-Resolved Fluorescence Assays)

Gyros (CD-based automated nanoliter microfluidic technology)

LC-MS, LBA/LC-MS

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Do Different Assays/Platforms Generate the Same Data?

Same sensitivity?

Same drug tolerance?

Same titers?

Same specificity?

Same detected seroconversion time (onset of ADA)?

→ NO

ADA assay life cycle - Assays need to be fit-for-purpose and fully validated in

support of phase 3

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