Module 9 2021

24/03/2021

Assay design & stats analysis

Common assay characteristics

A

• 96 well plate platform – most common

H

1

• Miniaturisation to 384 plates – on the increase

12

• Clear, black or white plates depending on endpoint measurement of assay: Abs, fluorescence, luminescence

The Organisation for Professionals in Regulatory Affairs

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Assay design & stats analysis

Common assay challenges

A

•

Edge effects • Row, column positional effects • Variation plate loading in assay – row A always first? • How long incubation steps at 37 o C (>18 hr) • Can lead to different responses from centre wells H 1

12

• Analyse whole plate precision to determine where positional effects are…

The Organisation for Professionals in Regulatory Affairs

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