Module 9 2021

24/03/2021

Whole plate precision analysis • A single conc. of substance prepared and delivered to the whole plate • Visualise where the plate response is not uniform and mitigate risk of positional plate bias • Always want to maximise the number of samples run per plate • Randomise plate layout to avoid positional bias Assay design & stats analysis

“hot” outer columns – plate in rows from opposite directions

“hot” outer wells – use inner 60 wells

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Assay design & stats analysis

• Use the whole 96 well plate

Assay Plate layout

A B C D

25000 6250 1562.5 390.63 97.66 24.41

6.1

1.53

0.38

0.1

0.02

0

2000 1000 2000 1000

500 357.14 255.1 182.22 130.15 92.97 66.41 47.43 11.86 2.96 500 357.14 255.1 182.22 130.15 92.97 66.41 47.43 11.86 2.96

200

100 0.02

50

35.71 25.51 18.22 13.02

9.3

6.64

4.74

1.19

0.3

0

0.1

0.38

1.53

6.1

24.41 97.66 390.63 1562.5 6250 25000

E F

2.96 11.86 47.43 66.41 92.97 130.15 182.22 255.1 357.14 500

1000 2000

0.3

1.19

4.74

6.64

9.3

13.02 18.22 25.51 35.71

50

100

200

G H

2.96 11.86 47.43 66.41 92.97 130.15 182.22 255.1 357.14 500

1000 2000

• or just the inner 60 well position

The Organisation for Professionals in Regulatory Affairs

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